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Publikasi Penelitian Dosen

PERBEDAAN POLA KROMATOGRAM DARI DAUN CENGKEH DAN BUNGA CENGKEH, Syzygium aromaticum (L.) Merr. & Perry., Myrtaceae.
Abstrak

PERBEDAAN POLA KROMATOGRAM DARI DAUN CENGKEH DAN BUNGA CENGKEH, Syzygium aromaticum (L.) Merr. & Perry., Myrtaceae. 

 

 

WIWI WINARTI. RATNA DJAMIL, EVA NURWAHIDAH

 

 

 

Fakultas Farmasi Universitas Pancasila, Srengseng Sawah, Jakarta 12640

 

 

 

Abstract : Clove buds and clove leaves (Syzygium aromaticum (L.) Merr. & Perry.) contain of volatile oil, but most people only use the buds for traditional medicine. Due to the statement above, author had made the research to isolated the volatile  oil  of  buds  and  leves  clove  by using  water  and  steam  distillation  then identified by using Thin Layer Chromatography (TLC) and Gas Chromatography – Mass Spectrometry (GC-MS). The result showed that there is a differences of total value from clove buds and clove leaves. Clove buds yield 12,94 per cent of volatile oil, containing 94,86 per cent of eugenol and clove leves yield 5,68 per cent of volatile oil, containing 98,30 per cent of eugenol. Meanwhile, the chromatogram based on the examination by using TLC and GC-MS from cloves buds and cloves leaf didn`t show any significant differences. Both of them have a same chemical constituent called eugenol.nevertheless, there are also the other different component on clove buds and clove leaves. The chemical constituent of clove buds that didn`t exist on clove leaves are Eugenyl Acetate, Methyl eugenol, and α-Humulene. On the opposite, the chemical constituent of clove leaves that didn`t exist on clove buds are

4,7,10-Cycloundecatriene, CaryophylleneOxyde, dan 3-Cyclohexen-1-ol,5-(2- butenylide).

 

 

Keywords  :  Clove buds  and  clove leaves    (Syzygium  aromaticum  (L.)  Merr. & Perry), volatile oil, water and steam distillation, Thin Layer Chromatography (TLC) and Gas Chromatography Mass Spectrometry (GC-MS).

 

 

Abstrak : Bunga cengkeh dan daun cengkeh (Syzygium aromaticum (L.) Merr. & Perry.) mengandung minyak atsiri, tetapi masyarakat pada umumnya menggunakan bunga cengkeh untuk obat tradisional. Berdasarkan hal tersebut diatas, penulis melakukan penelitian yang bertujuan untuk mengisolasi minyak atsiri yang berasal dari bunga dan daun cengkeh dengan metode destilasi uap dan air, kemudian minyak atsiri dari bunga dan daun cengkeh diidentifkasi secara Kromatografi Lapis Tipis dan Kromatografi Gas-Spektrometri Massa. Hasil penelitian menunjukkan perbedaan kadar total minyak atsiri yang berasal dari bunga cengkeh sebesar 12,94% dengan kandungan eugenol sebesar 94,86 %, dan kadar total minyak atsiri dari daun cengkesebesar 5,68% dengan kandungan eugenol sebesar 98,30 %. Sedangkan dari pemeriksaan Kromatografi Lapis Tipis dan Kromatografi Gas - Spektrometri Massa dari minyak atsiri daun dan bunga cengkeh diperoleh bercak kromatogram yang tidak jauh berbeda,

Penulis : RATNA DJAMIL , Dr MSi Apt [PDF File] didownload : 33 x

Penapisan Fitokimia, Uji BSLT, dan Uji Antioksidan Ekstrak Metanol beberapa Spesies Papilionaceae
Abstrak

Penapisan Fitokimia, Uji BSLT, dan Uji AntioksidanEkstrak Metanol beberapa Spesies Papilionaceae

 

 

 RATNA DJAMIL*, TRIA ANELIA

 Fakultas Farmasi Universitas Pancasila

 Jln. Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640.

 

 Diterima 20 Maret 2009,  Disetujui 24 Agustus 2009

 

 

 

Abstract: According to literatures, many plants of Papilionaceae family such as red bean (Phaseolus vulgaris L.), green bean (Phaseolus aureus Roxb.) and soybean (Glycine soja Sieb. & Zucc.) contain favonoid,  isofavonoid  and vitamin E. They all have antioxidant activity and are potential to reduce free radical. This research was aimed to identify secondary metabolites of crude powders and extracts of red bean, green bean and soybean. The bioactivity of each extracts was evaluated with Brine Shrimp Lethality Test (BSLT) method for preliminary assesment of toxicity. It was also evaluated for antioxidant activity using 1,1 difenil-2-pikrilhidrazil (DPPH) method. The results showed that red bean, green bean and soybean contain saponin, favonoid, steroid/triterpenoid, coumarin, and tannin. The result of BSLT showed that LC (Lethality Concentration 50) of red bean was 213.21 ppm; green bean 390.71 ppm;

and soybean 252.75 ppm. The result of DPPH tests showed that IC (Inhibition Concentration 50) of red bean was 47.54 ppm; green bean 95.08 ppm; and soybean 90.43 ppm.

 

 

Keywords: Papilionaceae, free radical, antioxidant, DPPH, BSLT.

 

 

Penulis : RATNA DJAMIL , Dr MSi Apt [PDF File] didownload : 34 x

Isolation of 8-glucopyranosylapigenine and anticancer activity from Anredera cordifolia (Ten.)Steenis
Abstrak

Isolation of 8-glucopyranosylapigenine and anticancer activity from Anredera cordifolia (Ten.)Steenis

 

Ratna Djamil1, Wahyudi PS2, Wahono S3, M.Hanafi 4

1Faculty of Pharmacy Pancasila University,Jakarta 12640,Indonesia

2Department of Chemistry,Faculty of Mathematics and Science, University of Indonesia,Depok 1624 Indonesia

3Agency for the Assessment and Application of Technology,Indonesia

4Research Centre for Chemistry, Indonesian Institute of Sciences

ratnadj_ffup@yahoo.co.id

 

 

 

Abstract

A flavonoid glycoside, 8-glucopyranosylapigenin were isolated from Anredera cordifolia

(Ten.)Steenis. The structures of the isolated compounds have been elucidated unambiguously by UV, MS, and a series of 1D and 2D NMR analyses. The isolated compounds  flavonoid glycoside, 8- glucopyranosylapigenin showed anticancer activity

 

 

Keyword: flavonoid glycoside, anticancer activity, Anredera cordifolia (Ten.)Steenis

Penulis : RATNA DJAMIL , Dr MSi Apt [PDF File] didownload : 26 x

Manajemen Risiko Dalam Pelayanan Farmasi di Rumah Sakit
Abstrak
Penulis : YUSI ANGGRIANI , Dr, M.Kes, Apt. [PDF File] didownload : 36 x

Clinical Outcomes Penguunaan Antibiotik pada pasien Infeksi Kaki Diabetik
Abstrak
Penulis : YUSI ANGGRIANI , Dr, M.Kes, Apt. [PDF File] didownload : 29 x

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