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2013-05-08 08:51:40
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2013-05-08 08:51:25
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2013-05-08 08:50:53
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Publikasi Penelitian Dosen

Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup (Annona muricata L) Extracts in LPS Stimulated-Macrophage Cell (RAW264.7)
Abstrak

Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup
(Annona muricata L) Extracts in LPS
Stimulated-Macrophage Cell (RAW264.7)
Dian Ratih Laksmitawati1, Ajeng Prima Prasanti1, Nadia Larasinta1, Gloria Agitha Syauta1, Rivanny Hilda1, Hesty Utami Ramadaniati1, Anisa Widyastuti1, Nadia Karami1, Merry Afni2,
Dwi Davidson Rihibiha2, Hanna Sari W. Kusuma2 and Wahyu Widowati3*
1Faculty of Pharmacy, University of Pancasila, Jakarta, Indonesia
2Biomoleculer and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
3Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia

Abstract
Inflammation is one of the important biological responses to injury. Anti-inflammatory is therefore proposed to treat both acute and chronic inflammation. Chemical compounds of various plants are widely used in treatment of inflammation. Objective: This study aims to evaluate anti-inflammatory potential of G. vulgaris extract (GVE) and A. muricata extract (AME) on LPS-stimulated murine macrophage cell line (RAW264.7). Cell viability assay to evaluate nontoxic concentration in cell line was performed with MTS assay. Parameters to determine anti-inflammatory activity between treatment group and non treated cells, were IL-1β, TNF-α, and IL-6 which was measured with Elisa, and NO level which was measured with nitrate/nitrite colorimetric assay. Both GVE and AME of 50 and 10 μg/mL showed high viability (>90%) and it was not significantly different compared to control, makes it suitable for treatment. GVE and AME of 50 μg/mL resulted low TNF-α level in RAW264.7(313.16pg/mL and 264.69 pg/mL respectively), as well as IL-1β level (903.53 pg/mL and 905.00 pg/mL respectively) and IL-6 (175.88 pg/mL and 219.13 pg/mL respectively). Whereas, GVE and AME of 75 μg/mL showed lower NO level (9.76 μM and 9.79 μM respectively) compared to untreated cells. This research revealed that GVE and AME possess the anti-inflammatory potential indicated by inhibition of inflammatory mediators including TNF-α, IL-1β, IL-6 and NO.

Keywords: Annona muricata L, Anti-inflammatory, Gendarussa vulgaris Nees, RAW264.7 Cell Line

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 19 x

Anti-inflammatory effects of Anredera cordifolia and Piper cro catum extracts on lipopolysaccharide-stimulated macrophage cell line
Abstrak

Anti-inflammatory effects of Anredera cordifolia and Piper crocatum
extracts on lipopolysaccharide-stimulated macrophage cell line
Dian Ratih Laksmitawati1, Anisa Widyastuti1, Nadia Karami1, Ervi Afifah1,
Dwi Davidson Rihibiha2, Hayatun Nufus2 and Wahyu Widowati3
1Faculty of Pharmacy, Pancasila University, Jalan Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia;
2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2, No. 9, Bandung
40163, West Java, Indonesia; 3Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri
No. 65 Bandung 40164, West Java, Indonesia.

Abstract
In this study, the anti-inflammatory potential of Anredera cordifolia and Piper
crocatum extracts on lipopolysaccharide-induced murine macrophage cell line
(RAW 264.7) was observed. Cell viability assay was performed with MTS
assay. Parameters measured to determine the anti-inflammatory activity were
interleukin-1􀇃 (IL-1􀇃), tumor necrosis factor (TNF)-􀇂, nitric oxide (NO) and IL
-6. Both A. cordifolia and P. crocatum at concentration of 50 μg/mL in cell line
resulted significant decrease in TNF-􀇂 level (250.3 and 242.5 pg/mL
respectively). A. cordifolia showed significant decrease in IL-1􀇃 level at 50 μg/
mL and IL-6 level at 10 μg/mL, whilst P. crocatum showed significant
decrease IL-1􀇃 level in three concentrations with lowest level at 50 μg/mL. A.
cordifolia showed lowest decrease in NO level at 50 μg/mL but not
comparable with normal cells, whilst P. crocatum showed significant decrease
in NO level at 50 μg/mL. This research revealed that A. cordifolia and P.
crocatum possess the anti-inflammatory potential indicated by the inhibitory
activity of the inflammatory mediators including, TNF-􀇂, IL-1􀇃, IL-6, and NO.

 

https://www.banglajol.info/index.php/BJP/article/view/28714 

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 19 x

Establishment Of Hyperuricemia Mouse Model With Oxonic Acid Potassium Salt And Essence Of Chicken
Abstrak

Establishment Of Hyperuricemia Mouse Model With Oxonic Acid Potassium Salt And Essence Of Chicken.
Dian Ratih Laksmitawati*, Liliek Nurhidayati, Nining Novita Ningsih.
Laboratorium Biochemsitry and Pharmacology Faculty of Pharmacy Pancasila University, Jalan Srengseng Sawah Jagakarsa Jakarta 12640
ABSTRACT
Nowadays the exploration of anti-hyperuricemia herbs becomes more interesting, but making an animal model of hyperuricemia is still facing a problem due to a different uric acid metabolism between mice and human. The aim of this study was to investigate the effects of essence of chicken (EC) and urease inhibitor, oxonic acid potassium salt (PO) used singly or in combination on the concentration of serum uric acid in mice, to determine the optimal procedure, time of blood withdrawal and induction period and to evaluate the feasibility of this method to establish a mouse model of hyperuricemia. Determination of blood withdrawal time was as follows: DDY mice was given PO 250 mg/kgBW intra peritoneally and the blood was collected per hour to measure the plasma uric acid concentration. Determination of inductor combination and period of induction time were conducted by dividing mice into 6 groups: normal, PO 1 day, PO 3 days, PO and EC 1 day, PO and EC 3 days and EC 3 day and PO only on the third day. On the third day, the blood was collected and plasma uric acid was reacted with EHSPT (N-Ethyl-N-(2-hydroxy-3-Sulfopropil) m-Toluidine) and 4-AAP(4-Aminoantipyrine). The absorbance of color formation were measured by using spectrophotometer at 546 nm. The results showed that plasma uric acid was the highest at 2 hours after OA injection. The administration of both PO and EC could raise the uric acid compared with singly OA. Administration of EC and PO combination each day for 3 days’ consecutively could increase plasma uric acid in mice. In conclusion, the administration of chicken essence (EC) with oxonic acid potassium salt (PO) for 3 days significantly increased the plasma uric acid. Thus, this procedure could be used as a suitable method to establish a mouse model of hyperuricemia.
Keywords: hyperuricemia model, potassium oxonic acid, essence of chicken.

 

https://www.rjpbcs.com/pdf/2017_8(1S)/[11].pdf

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 22 x

Presisi Uji Antihiperurisemia In Vitro Berdasarkan Pengukuran Serapan Pada Dua Panjang Gelombang
Abstrak

PRESISI UJI ANTIHIPERURISEMIA IN VITRO BERDASARKAN PENGUKURAN
SERAPAN PADA DUA PANJANG GELOMBANG
Liliek Nurhidayati, Dian Ratih Laksmitawati, Riska Eka Putri
Fakultas Farmasi, Universitas Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan
Corresponding author email: liliek_nurhidayati@yahoo.com
ABSTRAK
Metode yang digunakan dalam skrining obat antihiperurisemia in vitro berdasarkan pada
kemampuan suatu bahan menghambat enzim xantin oksidase dalam mengubah substrat xantin
menjadi asam urat. Para peneliti mengukur aktivitas antihiperurisemia berdasarkan asam urat
yang terbentuk atau xantin yang tersisa. Untuk mengetahui presisi kedua pengukuran tersebut,
telah dilakukan pengujian aktivitas antihiperurisemia alopurinol berdasarkan pengukuran
serapan pada panjang gelombang 291 nm dan 268 nm. Pada kondisi optimum diperoleh
simpangan baku relatif persen penghambatan berdasarkan jumlah asam urat yang terbentuk
0,24-1,30%, sedangkan berdasarkan sisa xantin adalah 0,25-2,39%.
Kata kunci : pengambatan, xantin oksidase, in vitro, alopurinol, presisi

http://kjif.unjani.ac.id/index.php/kjif/article/view/102 

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 21 x

Peningkatan Aktivitas Antimikroba Ekstrak Nanas (Ananas Comosus (L.). Merr) Dengan Pembentukan Nanopartikel
Abstrak

PENINGKATAN AKTIVITAS ANTIMIKROBA EKSTRAK NANAS (Ananas
comosus (L.). Merr) DENGAN PEMBENTUKAN NANOPARTIKEL
Deni Rahmat*, Dian Ratih L., Liliek Nurhidayati, Meilda Ayu Bathini
Fakultas Farmasi Universitas Pancasila, Jakarta
*Email : mangnden78@yahoo.com
ABSTRAK
Bonggol nanas (Ananas comosus (L.). Merr) mengandung enzim bromelain dengan kadar
tertinggi dibandingkan bagian nanas lainnya. Salah satu fungsi enzim bromelain adalah
sebagai antimikroba. Ekstrak bonggol nanas dibuat dalam bentuk nanopartikel dengan
menggunakan kitosan kemudian dikeringkan dengan metode freeze drying. Serbuk kering
ekstrak ditentukan Konsentrasi Hambat Minimum (KHM) terhadap Staphylococcus aureus.
Nanopartikel ekstrak kering dilakukan uji ukuran partikel, zeta potensial, dan morfologi
partikel. Nanopartikel ekstrak kemudian diformulasikan ke dalam bentuk sediaan gel. Tiap
formula dilakukan uji mutu fisik antara lain organoleptik, homogenitas, viskositas dan sifat
alir dan uji mutu kimia (pH). Hasil penelitian menunjukkan nanopartikel ekstrak memiliki
ukuran partikel 60,8 nm dan bentuk partikel kering sferis. Ekstrak bonggol nanas memiliki
KHM pada konsentrasi 1,25%. Gel formula IV menunjukkan DDH tertinggi yaitu 62,5 mm
dan memiliki viskositas dan pH yang stabil. Dengan demikian pembuatan nanopartikel pada
ekstrak bonggol nanas dapat meningkatkan aktivitas antimikroba dalam sediaan gel untuk
penggunaan secara topikal
Kata kunci: Bonggol nanas, nanopartikel, kitosan, antimikroba

https://jsk.farmasi.unmul.ac.id/index.php/jsk/article/view/45/40

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 19 x

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