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ISOLATION AND IDENTIFICATION OF A CHEMICAL COMPOUND IN 

n- HEXANE  PHASE FROM THE METHANOL EXTRACT OF BINAHONG LEAF (Anredera cordifolia (Ten.) Steenis), BASELLACEAE

Ratna Djamil1,2,3 , Wahyudi PS2, Wahono S3, Wiwi Winarti1, Evi Nurlativah1

1Faculty of Pharmacy Pancasila University,Jakarta 12640,Indonesia

2Department of Chemistry,Faculty of Mathematics and Science, University of Indonesia,Depok 1624 Indonesia

3Agency for the Assessment and Application of Technology, Indonesia (BPPT)

Email:ratnadj_ffup@yahoo.co.id

ABSTRACT:

Binahong (Anredera cordifolia (Ten.) Steenis) is one of the plant which is use generally for treatment of illness but no more research that explain about it’s chemical compound and efficacy. In this research had been done phytochemical screening, isolation and identification of chemical compound in n-heksana phase from methanol extract of that binahong’s leaves. Isolation doing by thin layer chromatography (TLC), vaccum liquid chromatography (VLC) and column chromatography (CC). Isolate was purified by TLC preparation and then two dimension TLC by using two eluens that has different polarity to know whether that isolate still had one spot at TLC plate. The chemical structure of isolate was identified by UV-Vis spectrophotometre, IR spectrophotometer, GC-MS. The result of phythochemical screening contains steroid, triterpenoid and volatile oil. The result of data’s analyses from UV-Vis, IR and GC-MS spectrum showed that isolate which had been identified given maxsimum absorbance at 230 nm has functional groups such as O-H, C-H, C-C, C=C and C-O with molecural weight 281. Based on data base Willey275.L assumed that chemical compound from n-heksana phase is 4-terpineol with 97% similarities.

Keywords : isolation, identification, binahong (Anredera cordifolia (Ten.) Steenis)

Apoptosis of human Breast Cancer Cells induced by

Ethylacetate Extracts of Propolis

1Syamsudin, 2Partomuan Simanjuntak, 3Ratna Djamil and 4Wan Lelly Heffen

1Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

2Department of Natural Product Chemistry, Research Centre for Biotechnology, Indonesian Institute of Science, Jln. Raya Bogor Km 46, Cibinong 16911, Indonesia

3Department of Research and Development, Dharmais Cancer Hospital, Jl. Letjen. S. Parman. Kav 84-86, Jakarta, Indonesia

4Department of Biological Pharmacy, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

Abstract: Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.

Key words: Apoptosis, propolis, human breast cancer cell

PERBEDAAN POLA KROMATOGRAM DARI DAUN CENGKEH DAN BUNGA CENGKEH, Syzygium aromaticum (L.) Merr. & Perry., Myrtaceae. 

WIWI WINARTI. RATNA DJAMIL, EVA NURWAHIDAH

Fakultas Farmasi Universitas Pancasila, Srengseng Sawah, Jakarta 12640

Abstract : Clove buds and clove leaves (Syzygium aromaticum (L.) Merr. & Perry.) contain of volatile oil, but most people only use the buds for traditional medicine. Due to the statement above, author had made the research to isolated the volatile  oil  of  buds  and  leves  clove  by using  water  and  steam  distillation  then identified by using Thin Layer Chromatography (TLC) and Gas Chromatography – Mass Spectrometry (GC-MS). The result showed that there is a differences of total value from clove buds and clove leaves. Clove buds yield 12,94 per cent of volatile oil, containing 94,86 per cent of eugenol and clove leves yield 5,68 per cent of volatile oil, containing 98,30 per cent of eugenol. Meanwhile, the chromatogram based on the examination by using TLC and GC-MS from cloves buds and cloves leaf didn`t show any significant differences. Both of them have a same chemical constituent called eugenol.nevertheless, there are also the other different component on clove buds and clove leaves. The chemical constituent of clove buds that didn`t exist on clove leaves are Eugenyl Acetate, Methyl eugenol, and α-Humulene. On the opposite, the chemical constituent of clove leaves that didn`t exist on clove buds are

4,7,10-Cycloundecatriene, CaryophylleneOxyde, dan 3-Cyclohexen-1-ol,5-(2- butenylide).

Keywords  :  Clove buds  and  clove leaves    (Syzygium  aromaticum  (L.)  Merr. & Perry), volatile oil, water and steam distillation, Thin Layer Chromatography (TLC) and Gas Chromatography – Mass Spectrometry (GC-MS).

Abstrak : Bunga cengkeh dan daun cengkeh (Syzygium aromaticum (L.) Merr. & Perry.) mengandung minyak atsiri, tetapi masyarakat pada umumnya menggunakan bunga cengkeh untuk obat tradisional. Berdasarkan hal tersebut diatas, penulis melakukan penelitian yang bertujuan untuk mengisolasi minyak atsiri yang berasal dari bunga dan daun cengkeh dengan metode destilasi uap dan air, kemudian minyak atsiri dari bunga dan daun cengkeh diidentifkasi secara Kromatografi Lapis Tipis dan Kromatografi Gas-Spektrometri Massa. Hasil penelitian menunjukkan perbedaan kadar total minyak atsiri yang berasal dari bunga cengkeh sebesar 12,94% dengan kandungan eugenol sebesar 94,86 %, dan kadar total minyak atsiri dari daun cengkeh sebesar 5,68% dengan kandungan eugenol sebesar 98,30 %. Sedangkan dari pemeriksaan Kromatografi Lapis Tipis dan Kromatografi Gas - Spektrometri Massa dari minyak atsiri daun dan bunga cengkeh diperoleh bercak kromatogram yang tidak jauh berbeda,

Keywords: Papilionaceae, free radical, antioxidant, DPPH, BSLT.