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DETERMINATION OF EUGENOL IN VOLATILE OIL FROM CLOVE LEAF AND FLOWER (Eugenia aromatica OK) BY TLC-DENSITOMETRY

Zuhelmi Aziz, Ratna Djamil, Desi Rahmi Suciati

Faculty of Pharmacy ,University of Pancasila

Srengseng Sawah, Jagakarsa, Jakarta 12640

email : zuhelmi_aziz@yahoo.com

ABSTRACT

Clove (Eugenia aromatic. OK ) is a tropical plant that come from Maluku. Clove oil contain volatile oil that usually obtained from flowers by the distillation method, but there are some literatures mentioned clove leaves also contain volatile oil. Based on this, research was conducted to determine the content of eugenol in volatile oil of clove leaf and flower. Determination of eugenol by TLC-Densitometry showed that, eugenol content in clove volatile oil from flower 82.49% and from leaf 79.03%.

Keywords: volatile oil , clove leaf and flower, eugenol, TLC-Densitometry.

ISOLATION AND IDENTIFICATION OF A CHEMICAL COMPOUND IN 

n- HEXANE  PHASE FROM THE METHANOL EXTRACT OF BINAHONG LEAF (Anredera cordifolia (Ten.) Steenis), BASELLACEAE

Ratna Djamil1,2,3 , Wahyudi PS2, Wahono S3, Wiwi Winarti1, Evi Nurlativah1

1Faculty of Pharmacy Pancasila University,Jakarta 12640,Indonesia

2Department of Chemistry,Faculty of Mathematics and Science, University of Indonesia,Depok 1624 Indonesia

3Agency for the Assessment and Application of Technology, Indonesia (BPPT)

Email:ratnadj_ffup@yahoo.co.id

ABSTRACT:

Binahong (Anredera cordifolia (Ten.) Steenis) is one of the plant which is use generally for treatment of illness but no more research that explain about it’s chemical compound and efficacy. In this research had been done phytochemical screening, isolation and identification of chemical compound in n-heksana phase from methanol extract of that binahong’s leaves. Isolation doing by thin layer chromatography (TLC), vaccum liquid chromatography (VLC) and column chromatography (CC). Isolate was purified by TLC preparation and then two dimension TLC by using two eluens that has different polarity to know whether that isolate still had one spot at TLC plate. The chemical structure of isolate was identified by UV-Vis spectrophotometre, IR spectrophotometer, GC-MS. The result of phythochemical screening contains steroid, triterpenoid and volatile oil. The result of data’s analyses from UV-Vis, IR and GC-MS spectrum showed that isolate which had been identified given maxsimum absorbance at 230 nm has functional groups such as O-H, C-H, C-C, C=C and C-O with molecural weight 281. Based on data base Willey275.L assumed that chemical compound from n-heksana phase is 4-terpineol with 97% similarities.

Keywords : isolation, identification, binahong (Anredera cordifolia (Ten.) Steenis)

Apoptosis of human Breast Cancer Cells induced by

Ethylacetate Extracts of Propolis

1Syamsudin, 2Partomuan Simanjuntak, 3Ratna Djamil and 4Wan Lelly Heffen

1Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

2Department of Natural Product Chemistry, Research Centre for Biotechnology, Indonesian Institute of Science, Jln. Raya Bogor Km 46, Cibinong 16911, Indonesia

3Department of Research and Development, Dharmais Cancer Hospital, Jl. Letjen. S. Parman. Kav 84-86, Jakarta, Indonesia

4Department of Biological Pharmacy, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

Abstract: Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.

Key words: Apoptosis, propolis, human breast cancer cell