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Apoptosis of human Breast Cancer Cells induced by

Ethylacetate Extracts of Propolis

1Syamsudin, 2Partomuan Simanjuntak, 3Ratna Djamil and 4Wan Lelly Heffen

1Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

2Department of Natural Product Chemistry, Research Centre for Biotechnology, Indonesian Institute of Science, Jln. Raya Bogor Km 46, Cibinong 16911, Indonesia

3Department of Research and Development, Dharmais Cancer Hospital, Jl. Letjen. S. Parman. Kav 84-86, Jakarta, Indonesia

4Department of Biological Pharmacy, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, Indonesia

Abstract: Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.

Key words: Apoptosis, propolis, human breast cancer cell

PERBEDAAN POLA KROMATOGRAM DARI DAUN CENGKEH DAN BUNGA CENGKEH, Syzygium aromaticum (L.) Merr. & Perry., Myrtaceae. 

WIWI WINARTI. RATNA DJAMIL, EVA NURWAHIDAH

Fakultas Farmasi Universitas Pancasila, Srengseng Sawah, Jakarta 12640

Abstract : Clove buds and clove leaves (Syzygium aromaticum (L.) Merr. & Perry.) contain of volatile oil, but most people only use the buds for traditional medicine. Due to the statement above, author had made the research to isolated the volatile  oil  of  buds  and  leves  clove  by using  water  and  steam  distillation  then identified by using Thin Layer Chromatography (TLC) and Gas Chromatography – Mass Spectrometry (GC-MS). The result showed that there is a differences of total value from clove buds and clove leaves. Clove buds yield 12,94 per cent of volatile oil, containing 94,86 per cent of eugenol and clove leves yield 5,68 per cent of volatile oil, containing 98,30 per cent of eugenol. Meanwhile, the chromatogram based on the examination by using TLC and GC-MS from cloves buds and cloves leaf didn`t show any significant differences. Both of them have a same chemical constituent called eugenol.nevertheless, there are also the other different component on clove buds and clove leaves. The chemical constituent of clove buds that didn`t exist on clove leaves are Eugenyl Acetate, Methyl eugenol, and α-Humulene. On the opposite, the chemical constituent of clove leaves that didn`t exist on clove buds are

4,7,10-Cycloundecatriene, CaryophylleneOxyde, dan 3-Cyclohexen-1-ol,5-(2- butenylide).

Keywords  :  Clove buds  and  clove leaves    (Syzygium  aromaticum  (L.)  Merr. & Perry), volatile oil, water and steam distillation, Thin Layer Chromatography (TLC) and Gas Chromatography – Mass Spectrometry (GC-MS).

Abstrak : Bunga cengkeh dan daun cengkeh (Syzygium aromaticum (L.) Merr. & Perry.) mengandung minyak atsiri, tetapi masyarakat pada umumnya menggunakan bunga cengkeh untuk obat tradisional. Berdasarkan hal tersebut diatas, penulis melakukan penelitian yang bertujuan untuk mengisolasi minyak atsiri yang berasal dari bunga dan daun cengkeh dengan metode destilasi uap dan air, kemudian minyak atsiri dari bunga dan daun cengkeh diidentifkasi secara Kromatografi Lapis Tipis dan Kromatografi Gas-Spektrometri Massa. Hasil penelitian menunjukkan perbedaan kadar total minyak atsiri yang berasal dari bunga cengkeh sebesar 12,94% dengan kandungan eugenol sebesar 94,86 %, dan kadar total minyak atsiri dari daun cengkeh sebesar 5,68% dengan kandungan eugenol sebesar 98,30 %. Sedangkan dari pemeriksaan Kromatografi Lapis Tipis dan Kromatografi Gas - Spektrometri Massa dari minyak atsiri daun dan bunga cengkeh diperoleh bercak kromatogram yang tidak jauh berbeda,

Keywords: Papilionaceae, free radical, antioxidant, DPPH, BSLT.

Isolation of 8-glucopyranosylapigenine and anticancer activity from Anredera cordifolia (Ten.)Steenis

Ratna Djamil1, Wahyudi PS2, Wahono S3, M.Hanafi 4

1Faculty of Pharmacy Pancasila University,Jakarta 12640,Indonesia

2Department of Chemistry,Faculty of Mathematics and Science, University of Indonesia,Depok 1624 Indonesia

3Agency for the Assessment and Application of Technology,Indonesia

4Research Centre for Chemistry, Indonesian Institute of Sciences

ratnadj_ffup@yahoo.co.id

Abstract

A flavonoid glycoside, 8-glucopyranosylapigenin were isolated from Anredera cordifolia

(Ten.)Steenis. The structures of the isolated compounds have been elucidated unambiguously by UV, MS, and a series of 1D and 2D NMR analyses. The isolated compounds  flavonoid glycoside, 8- glucopyranosylapigenin showed anticancer activity

Keyword: flavonoid glycoside, anticancer activity, Anredera cordifolia (Ten.)Steenis