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2013-05-08 08:51:40
Untag Telah Luluskan 35.000 Sarjana
2013-05-08 08:51:25
FKIP Untirta Gelar Gebyar Hardiknas 2013
2013-05-08 08:50:53
Hasil penelitian wajib disertai sosialisasi

Publikasi Penelitian Dosen

Isolation and identification of a chemical compounds in n-hexane phase from the methanol extract of keladi tikus leaf (Typhonium flagelliforme Lodd. Blume, Araceae
Abstrak

Abstract: Keladi tikus (Typhonium flagelliforme (Lodd.) Blume ) is a plant which empirically has efficacy in treating cancer. In this study, n-hexane phase was fractionated using Vacuum Liquid Chromatography (dichloromethane; isopropanol 98:2 (1:1) and Column Chromatography (SiO2, n-hexane-ethylacetate =3:1) and then each fraction was tested for toxicity with BSLT (brine shrimp lethality test.) method. The most active fraction was purified by TLC preparative and the pure compound was identified by using UV-Vis Spectrophotometer, IR Spectrophotometer, and Gas Chromatography-Mass Spectrometer. The result showed that fraction H.2.5 was the most active fraction with percent mortality 90% and one of the compounds contained in the n-hexane phase of keladi tikus leaves is phytol compound that had 91% similarity with the molecular formula C20H40O and the molecular weight of 296.31 

Keywords : isolation,  keladi tikus, Typhonium flagelliforme (Lodd). Blume, BSLT

Penulis : YUNAHARA FARIDA , Dr [PDF File] didownload : 48 x

Uji aktivitas antioksidan dan uji sitotoksik ekstrak n-heksana dan ekstrak metanol daun keladi tikus (Typhonium divaricatum L.Decne)
Abstrak

Cytotoxic and antioxidant activities of Typhonium divaricatum (L) Decne leaves extract have been explored. Preliminary phytochemical screening on the extract were carried out for the compound's profile. Biological activity was measured by using Brine Shrimp Lethality Test (BSLT), while cytotoxic activity was conducted on breast cancer cell line T-47D using MTT method. Antioxidant activity was measured by using DPPH free radical scavenging method. The results of phytochemical screening showed that the methanol extract contained flavonoid, saponin and steroid/triterpenoid compounds, meanwhile the n-hexane extract contained steroid/triterpenoid compounds only. The highest biological activity was found in methanol extract (LC50 =32,9 ppm). The highest cytotoxic activity was shown by n-hexane extract (32,50 ppm). The highest antioxidant activity using vitamin C as positive control was found in methanol extract (IC50 = 56,63 ppm). The preliminary results indicated that Typhonium divaricatum (L) Decne leave is potential  potential for anticancer and free radical scavenger.

Keywords: Typhonium divaricatum (L) Decne, BSLT, Cytotoxic activity, T-47D, MTT, DPPH

Penulis : YUNAHARA FARIDA , Dr [PDF File] didownload : 66 x

Physical Characteristics of Capsule of keladi tikus extract (Typhonium divaricatum L.Decne) from spray drying method using maltodextrine
Abstrak

Keladi tikus (Typhonium divaricatum (L.) Decne), one of many wild plants, are consumed by cancer patients. Keladi tikus have antioxidant activity which can resist the effects of free radicals. This research including phytochemical screening using the Farnsworth method, a test of antioxidant activity using DPPH free radical reduction (1.1-diphenyl-2-pikrilhidrazil), drying of keladi tikus extracts (n-hexane and methanol) by spray drying method and formulation granule extract into a capsule dosage form have been evaluated. Concentration of maltodextrin as filler used for the spray drying process were 35%, 40% and 45% for n-hexane extract and 40%, 45% and 55% for methanol extract. Capsules evaluated weight uniformity, disintegration time and dissolution rate. The results showed that the phytochemical screening of methanol extracts indicate flavonoids, saponins and steroids / triterpenoid, in n-hexane extract showed the presence of steroid / triterpenoid. Antioxidant activity by DPPH test showed IC50 values of the extract methanol was 56,63 ppm. Results of uniform weight of capsule were as follows formula I-III: 0,3718 ± 0,0034 g-0, 3673 ± 0,0039 g and formula IV-VI 0,3783 ± 0,0053 g -0, 3812 ± 0, 0047 g. Disintegration time of capsule was formula I-III: 6.57 - 8.13 minutes and formula IV-VI: 7.80 - 9.19 minutes. Dissolution rate of capsule showed that the concentration of maltodextrin increased as the increasing of dissolution rate capsule. Based on one-way analysis ANVA of disintegration time and dissolution rate with variation concentration of maltodextrin concluded that there was a statistically significant difference.

 

Keywords: Capsule dosage form, Physical characteristic, Keladi tikus extract,  Maltodextrine, Spray drying

Penulis : YUNAHARA FARIDA , Dr [PDF File] didownload : 36 x

Perbandingan spektrum KLT-densitometri dari ekstrak etanol jahe (Zingiber officinale Rosc.) dengan pelarut etanol yang berbeda konsentrasinya,
Abstrak

Jahe (Zingiber officinale Rosc.) telah lama dimanfaatkan secara empiris oleh masyarakat Indonesia sebagai obat tradisional. Pada saat ini industri obat tradisional mulai mengembangkan tanaman yang berkhasiat obat tidak saja dalam bentuk serbuk simplisia tetapi sudah dibuat dalam bentuk ekstrak. Pada pembuatan ekstrak, industri obat tradisional menggunakan pelarut yang tidak berbahaya seperti etanol. Penelitian ini bertujuan membandingkan spektrum serapan secara KLT densitometri rimpang jahe yang diekstraksi menggunakan etanol dengan konsentrasi 50, 70 dan 96%. Masing-masing ekstrak kemudian dikromatografi dengan eluasi dua kali berturut-turut menggunakan cairan eluasi pertama : toluen-etil asetat-asam format (90:8:2) dan eluasi kedua : toluen-etil asetat = 90:10. Kromatogram yang diperoleh diperiksa spektrumnya secara KLT densitometri pada panjang gelombang 217, 254, 284 dan 366 nm. Spektrum yang diperoleh dibandingkan untuk melihat persamaan dan perbedaannya. Hasil penelitian menunjukkan ekstraksi dengan etanol 50% menghasilkan rendemen yang lebih besar dibandingkan  etanol 70 dan 96%. Dari hasil KLT densitometri menunjukkan ekstrak etanol 96% memberikan bercak yang lebih banyak dibandingkan ekstrak etanol 50 dan 70%.

Penulis : YUNAHARA FARIDA , Dr [PDF File] didownload : 81 x

Cytotoxic Activity of Ocimum americanum L. Leaves Against T47D Breast Cancer Cells using MTT Assay
Abstrak

Abstract: This is an advance experiment of the previous cytotoxic screening test using BSLB method which resulted that methanol extract of Ocimum americanum L. leaves has the highest cytotoxicity potential. In this experiment, extraction of Ocimum americanum L. leaves have been conducted by maceration using  n-hexane, ethyl acetate, and methanol solvent. These extracts are then tested for their cytotoxic activity. The purpose of this experiment is to detect the cytotoxic activitiy of various extract of Ocimum americanum L. leaves against T47D breast cancer cells in vitro. The MTT assay method was used with fraction concentration test series of 10, 20, 40, and 80 µg/mL. LC50 value was used as a parameter for cytotoxicity and obtained by probit analysis. National Cancer Institute recommended that any extracts generates LC50 below than 30 µg/mL is considered to possess cytotoxic activity. The results indicate that LC50 of n-hexane extract of Ocimum americanum L. leaves could not be determined in sampel concentrations of ≤ 80 µg/mL. Whilst the LC50 value can be determined in the other two extracts. The LC50 value of ethyl acetate and methanol extract of Ocimum americanum L. leaves were 26.7 µg/mL and 276 µg/mL, respectively. It can be concluded that ethyl acetate extract of Ocimum americanum L. has potential  cytotoxic activity against T47D breast cancer cells. 

 

Keywords: cytotoxic, extract, MTT assay, Ocimum americanum L., T47D, LC50.                               

 

 

Penulis : SESILIA ANDRIANI K, MSI., APT. [PDF File] didownload : 32 x

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