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2013-05-08 08:51:40
Untag Telah Luluskan 35.000 Sarjana
2013-05-08 08:51:25
FKIP Untirta Gelar Gebyar Hardiknas 2013
2013-05-08 08:50:53
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Publikasi Penelitian Dosen

Subchronic Toxicity Effect of Formalin Residue in Fish on The Mouse Liver
Abstrak

Subchronic Toxicity Effect of Formalin Residue in Fish on The Mouse Liver

Jovita Tri Murtini, Yeni Pratiwi Puspitasari, Ros Sumarny

 

Abstract

Research on subchronic toxicity effect of formalin residue in fish on the mouse liver has been done. This research was aimed to give scientific explanation concerning illegal application of formalin in food products, including fish. Formalin is harmful and thereby is banned to be used as perservative agent. However, in some places formalin is apllied to extend the shelf life of either fresh or processed  fish products. In term of the research treatments, the mice were divided into four groups : negative control, 1st dosage  (formalin residue 3 ppm), 2nd dosage (formalin residue 6 ppm), 3rd dosage  (formalin residue 12 ppm), each group was tested on 18 mice. Blood and histopatology analysis were conducted every 2 weeks for 6 weeks. The research resulted that GOT concentration highly increased in the 2nd week and GPT concentration highly increased in the 4th week, while there was no decreasing GPT and GOT concentration during 2 weeks recovery. Increasing formalin concentration in fish meal caused the increase of GOT and GPT level. The histopatology analysis showed that mouse liver cell degerenated in the  2nd week at the residue of 3 ppm and necroted at formalin residue of 6 ppm and 12 ppm. After 2 weeks recovery, there was no indication of mouse liver recovery.

 

Keywords: subchronic toxicity, formalin residue, fish mouse liver.  

Penulis : ROS SUMARNY , Prof. Dr., MS, Apt [PDF File] didownload : 58 x

Penghambatan Ekspresi Gen dengan Antisense Oligonukleotida sebagai Upaya
Abstrak

Due to the development of biomolecular science eg.overexpression genes, genes that cause diseases can be identified. Based on this fact, researchers developed a therapeutic strategy by inhibiting the gene expression using oligonucleotide antisense. Therapy using oligonucleotide antisense was based on a natural process of gene expression. Spesific artificial antisense will match complementary with DNA and mRNA. By this process the transcription will stop. The effort of therapy is relatively new but a few have been cartried out in clinical trial phase. Obstacles are encountered in reaching the target cell by oligonucleotide antisense.

Keyword : oligonucleotide, antisense, gene expression, therapy

 

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 81 x

Secretion of Indoleamine 2,3-Dioxygenase, An Immunomodulatory Substance, By adipose-Derived Mesenchymal Stem Cell
Abstrak

Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant adipose-derived-mesenchymal stem cells (MSCs). Mesenchymal stem cells have been studied in many research areas to regenerate many cell lineages. In addition, MSCs have immunomodulatory effect. This capability has been utilized in several clinical studies in hematopoetic stem cell and organ transplantation as a strategy to reduce the risk of Graft versus Host Disease (GvHD). It has been reported that the ‘stimulated’ MSC is able to secrete substances to suppress tissue rejection. One of the substances was known to be indoleamine 2,3-dioxygenase (IDO).  A previous study has characterized the secretion of IDO by bone marrow-derived MSCs stimulated by an inflammatory mediator interferon gamma (IFNγ). Indoleamine 2,3-dioxygenase has been detected using Western blot analysis and by High Performance Liquid Chromatography (HPLC) assay. The aim of this study was to detect the presence of IDO in adipose-derived MSCs culture with and without INFγ stimulation. Our study showed that adipose-derived-MSC stimulated with IFN γ significantly secreted high level of IDO as detected by Enzyme-Linked Immuno Sorbent Assay (ELISA). Despite its property as a proinflammatory mediator, IFN γ has shown to be able to induce IDO secretion in MSC culture which suggests the immuno modulatory effect of MSC. This study clearly demonstrates the potential application of adipose-derived MSC in the immunomodulatory strategy for allogenic transplantation. 

 

Keyword : mesenchymal stem cell, indoleamine 2,3-dioxygenase, IFNγ

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 60 x

Blood Lipids And Oxidative Stress In β-thalassemia Patients In Jakarta
Abstrak

A study on thalassemia patients in Jakarta was initiated to obtain a comprehensive of metabolic dysregulation, iron overload, oxidative stress, and cell damaged. This paper focuses on blood lipids from transfusion-dependent patients in an age range of 11-25 years (T) and another group of 9 frequently transfused (for at least 15 years) patiens aged 17-30 years (L). A third group comprises 6 patients (aged 4- 14 years) who had not yet obtained transfusions. The 20 controls (C) were voluntary students without diagnosis or clinical signs of thalassemia up to 30 years of age. The hematological results can be summarized that non-transfused thalassemia intermedia patients exert slight signs of oxidative stress, and increased hemoglobin degradation but no significant indication of tissue or cell damage. This picture differs considerably from transfusion-dependent thalassemia major patients and is even worse in long-term transfused patients : higly significant decrease in antixidants and thiols and tremendous iron overload and cell damage. Total cholesterol, HDL, and LDL are decreased, whereas triglyserides and ratios of total cholesterol or LDL to HDL are higher than in controls. Malondialdehyde, a byproduct of lipid peroxidation increases from controls <  N < T < L. The relation between plasma and red cell membranes is discussed.

 

Keyword : Thalasemia, blood lipid, oxidative stress

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 49 x

Iron Status and oxidative stress in β-thalassemia patients in Jakarta
Abstrak

A study on thalassemia intermedia and major patients in Jakarta was initiated to obtain a comprehensive picture of metabolic dysregulation, iron overload, oxidative stress, and cell damage. Data are presented from a group of 14 transfusion-dependent patients in an age range of 11-25 years (T) and another group of 9 frequently transfused (for at least 15 years) patients aged 17-30 years (L). A third group comprises 6 patients (aged 7 to 14 years) who had not yet obtained transfusions (N). The 21 controls (C) were voluntary students without diagnosis or clinical signs of thalassemia up to 30 years of age. The study was approved by the Ethical Clearance Board of the Medical Faculty and all blood samples from controls and patients were obtained on fully informed consent. Levels of antioxidants (vitamins A, C, E and b-carotene) and reactive thiols are considerably decreased in transfused patients, whereas signs of iron overload and cell damage are increased (serum iron, ferritin, transferrin saturation, SGOT, SGPT, g-GT, bilirubin). Results can be summarized that non-transfused thalassemia intermedia patients exert slight signs of oxidative stress, and increased hemoglobin degradation but no significant indication of tissue or cell damage. This picture differs considerably from transfusion-dependent thalassemia major patients: highly significant decrease in antioxidants and thiols and tremendous iron overload and cell damage. The picture is even worsened in long-term transfused patients. Iron chelation after transfusion is not sufficient in Indonesia, because it is normally (with few exceptions) applied only once together with transfusion. Hence, one major reason of the bad condition of transfusion-dependent thalassemia patients in Indonesia appears to be frequent transfusions (on the average one per month) and insufficient chelation of one treatment per month together with transfusion.

Keyword :Thalassemia major, thalassemia intermedia, iron status, transfusion, oxidative stress.

 

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 63 x

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