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Metode yang digunakan dalam skrining obat antihiperurisemia in vitro berdasarkan pada kemampuan suatu bahan menghambat enzim xantin oksidase dalam mengubah substrat xantin menjadi asam urat. Para peneliti mengukur aktivitas antihiperurisemia berdasarkan asam urat yang terbentuk atau xantin yang tersisa. Untuk mengetahui presisi kedua pengukuran tersebut, telah dilakukan pengujian aktivitas antihiperurisemia alopurinol berdasarkan pengukuran serapan pada panjang gelombang 291 nm dan 268 nm. Pada kondisi optimum diperoleh simpangan baku relatif prosen penghambatan berdasarkan jumlah asam urat yang terbentuk 0,29-2,65%, sedangkan berdasarkan sisa xantin adalah 0,37-2,31%.

Kata kunci : pengambatan, xantin oksidase, in vitro, alopurinol, presisi  

Garcinia mangostana L rind contains xanthones which are not soluble in water. One of the type of xanthones is α-mangosteen that has a high antioxidant activity. To improve its solubility, in this research, cosolvent addition method was used. The xanthones were extracted from mangosteen rind using 70% ethanol and then formulated into oral solution by dissolving the extract with various cosolvents and addition of sorbitol and water soluble violet dye. The composition of cosolvents were PEG 400-glycerol (20:20, 20:40, 40:20, 40:40). The clarity, pH and density of all the oral solution were fulfill the requirement. Oral solution with cosolvent of PEG 400-glycerol (40:40) has the highest content of α-mangosteen. 

Keyword : Mangosteen rind, α-mangosteen, antioxidant, cosolvency