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Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear
Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed
Intracellular Reactive Oxygen Species Level of Peripheral Blood-derived
Endothelial Progenitor Cells
Wahyu Widowati1,*, Laura Wijaya2, Dian Ratih Laksmitawati3, Rahma Micho Widyanto4,
Pande Putu Erawijantari5, Nurul Fauziah5, Indra Bachtiar2, and Ferry Sandra6,7,*
1Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia
2Stem Cell and Cancer Institute, Jakarta 13210, Indonesia
3Faculty of Pharmacy, Pancasila University, Jakarta 12640, Indonesia
4Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia
5Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
6Faculty of Dentistry, Trisakti University, Jakarta 11440, Indonesia
7Prodia Clinical Laboratory, Jakarta 10430, Indonesia
Abstract − Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be
reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 μmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
Keywords − Tea flavonoids, Antioxidant, Endothelial progenitor cell, Differentiation, ROS, Apoptosis

http://dx.doi.org/10.20307/nps.2016.22.2.87 

https://www.researchgate.net/publication/305461566_Tea_Flavonoids_Induced_Differentiation_of_Peripheral_Blood-derived_Mononuclear_Cells_into_Peripheral_Blood-derived_Endothelial_Progenitor_Cells_and_Suppressed_Intracellular_Reactive_Oxygen_Species_Lev

In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW264.7 murine macrophage cell line
Ni Made Dwi Sandhiutami 1, Moordiani Moordiani 1, Dian Ratih Laksmitawati 1, Nurul Fauziah 2, Maesaroh Maesaroh 2, Wahyu Widowati 3*
1 Faculty of Pharmacy, University of Pancasila, Jl. Srenseng Sawah, Jagakarsa, Jakarta 12640, Indonesia
2 Bimolecular and Biomedical Research Center, Aretha Medika Utama,Jl. Babakan Jeruk 2 no 9, Bandung 40163, Indonesia
3 Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Suria Sumantri no 65 Bandung 40164, Indonesia

A B S T R A C T

Objective(s): Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer’s and various cancer. Indonesian cassia (Cinnamomum burmannil C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an anti-inflammatory. This study was conducted to determine the anti-inflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW264.7 cell line.
Materials and Methods: The cytotoxic assay of coumarin and ICE against RAW264.7 cells was conducted using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW 267.4 macrophages cells to measure inhibitory activity of compound and ISEon production of nitric oxide (NO), prostaglandin E2 (PGE2), and also cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α.
Results: Coumarin 10 μM and ICE 10 μg/ml were nontoxic to the RAW264.7 cells. Both of coumarin and ICE were capable to reduce the PGE2, TNF-α, NO, IL-6, and IL-β level in LPS-induced RAW264.7 cells. Coumarin had higher activity to decrease PGE2 and TNF-α, whilst ICE had higher activity to inhibit NO, IL-6, and IL-β levels.
Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE2 and NO along with pro-inflammatory cytokines TNF-α, IL-6, IL-1β production.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243982/pdf/IJBMS-20-99.pdf

Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup
(Annona muricata L) Extracts in LPS
Stimulated-Macrophage Cell (RAW264.7)
Dian Ratih Laksmitawati1, Ajeng Prima Prasanti1, Nadia Larasinta1, Gloria Agitha Syauta1, Rivanny Hilda1, Hesty Utami Ramadaniati1, Anisa Widyastuti1, Nadia Karami1, Merry Afni2,
Dwi Davidson Rihibiha2, Hanna Sari W. Kusuma2 and Wahyu Widowati3*
1Faculty of Pharmacy, University of Pancasila, Jakarta, Indonesia
2Biomoleculer and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
3Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia

Abstract
Inflammation is one of the important biological responses to injury. Anti-inflammatory is therefore proposed to treat both acute and chronic inflammation. Chemical compounds of various plants are widely used in treatment of inflammation. Objective: This study aims to evaluate anti-inflammatory potential of G. vulgaris extract (GVE) and A. muricata extract (AME) on LPS-stimulated murine macrophage cell line (RAW264.7). Cell viability assay to evaluate nontoxic concentration in cell line was performed with MTS assay. Parameters to determine anti-inflammatory activity between treatment group and non treated cells, were IL-1β, TNF-α, and IL-6 which was measured with Elisa, and NO level which was measured with nitrate/nitrite colorimetric assay. Both GVE and AME of 50 and 10 μg/mL showed high viability (>90%) and it was not significantly different compared to control, makes it suitable for treatment. GVE and AME of 50 μg/mL resulted low TNF-α level in RAW264.7(313.16pg/mL and 264.69 pg/mL respectively), as well as IL-1β level (903.53 pg/mL and 905.00 pg/mL respectively) and IL-6 (175.88 pg/mL and 219.13 pg/mL respectively). Whereas, GVE and AME of 75 μg/mL showed lower NO level (9.76 μM and 9.79 μM respectively) compared to untreated cells. This research revealed that GVE and AME possess the anti-inflammatory potential indicated by inhibition of inflammatory mediators including TNF-α, IL-1β, IL-6 and NO.

Keywords: Annona muricata L, Anti-inflammatory, Gendarussa vulgaris Nees, RAW264.7 Cell Line

Anti-inflammatory effects of Anredera cordifolia and Piper crocatum
extracts on lipopolysaccharide-stimulated macrophage cell line
Dian Ratih Laksmitawati1, Anisa Widyastuti1, Nadia Karami1, Ervi Afifah1,
Dwi Davidson Rihibiha2, Hayatun Nufus2 and Wahyu Widowati3
1Faculty of Pharmacy, Pancasila University, Jalan Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia;
2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2, No. 9, Bandung
40163, West Java, Indonesia; 3Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri
No. 65 Bandung 40164, West Java, Indonesia.

Abstract
In this study, the anti-inflammatory potential of Anredera cordifolia and Piper
crocatum extracts on lipopolysaccharide-induced murine macrophage cell line
(RAW 264.7) was observed. Cell viability assay was performed with MTS
assay. Parameters measured to determine the anti-inflammatory activity were
interleukin-1􀇃 (IL-1􀇃), tumor necrosis factor (TNF)-􀇂, nitric oxide (NO) and IL
-6. Both A. cordifolia and P. crocatum at concentration of 50 μg/mL in cell line
resulted significant decrease in TNF-􀇂 level (250.3 and 242.5 pg/mL
respectively). A. cordifolia showed significant decrease in IL-1􀇃 level at 50 μg/
mL and IL-6 level at 10 μg/mL, whilst P. crocatum showed significant
decrease IL-1􀇃 level in three concentrations with lowest level at 50 μg/mL. A.
cordifolia showed lowest decrease in NO level at 50 μg/mL but not
comparable with normal cells, whilst P. crocatum showed significant decrease
in NO level at 50 μg/mL. This research revealed that A. cordifolia and P.
crocatum possess the anti-inflammatory potential indicated by the inhibitory
activity of the inflammatory mediators including, TNF-􀇂, IL-1􀇃, IL-6, and NO.

https://www.banglajol.info/index.php/BJP/article/view/28714 

Establishment Of Hyperuricemia Mouse Model With Oxonic Acid Potassium Salt And Essence Of Chicken.
Dian Ratih Laksmitawati*, Liliek Nurhidayati, Nining Novita Ningsih.
Laboratorium Biochemsitry and Pharmacology Faculty of Pharmacy Pancasila University, Jalan Srengseng Sawah Jagakarsa Jakarta 12640
ABSTRACT
Nowadays the exploration of anti-hyperuricemia herbs becomes more interesting, but making an animal model of hyperuricemia is still facing a problem due to a different uric acid metabolism between mice and human. The aim of this study was to investigate the effects of essence of chicken (EC) and urease inhibitor, oxonic acid potassium salt (PO) used singly or in combination on the concentration of serum uric acid in mice, to determine the optimal procedure, time of blood withdrawal and induction period and to evaluate the feasibility of this method to establish a mouse model of hyperuricemia. Determination of blood withdrawal time was as follows: DDY mice was given PO 250 mg/kgBW intra peritoneally and the blood was collected per hour to measure the plasma uric acid concentration. Determination of inductor combination and period of induction time were conducted by dividing mice into 6 groups: normal, PO 1 day, PO 3 days, PO and EC 1 day, PO and EC 3 days and EC 3 day and PO only on the third day. On the third day, the blood was collected and plasma uric acid was reacted with EHSPT (N-Ethyl-N-(2-hydroxy-3-Sulfopropil) m-Toluidine) and 4-AAP(4-Aminoantipyrine). The absorbance of color formation were measured by using spectrophotometer at 546 nm. The results showed that plasma uric acid was the highest at 2 hours after OA injection. The administration of both PO and EC could raise the uric acid compared with singly OA. Administration of EC and PO combination each day for 3 days’ consecutively could increase plasma uric acid in mice. In conclusion, the administration of chicken essence (EC) with oxonic acid potassium salt (PO) for 3 days significantly increased the plasma uric acid. Thus, this procedure could be used as a suitable method to establish a mouse model of hyperuricemia.
Keywords: hyperuricemia model, potassium oxonic acid, essence of chicken.

https://www.rjpbcs.com/pdf/2017_8(1S)/[11].pdf