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Penapisan Fitokimia, Uji BSLT, dan Uji AntioksidanEkstrak Metanol beberapa Spesies Papilionaceae

 RATNA DJAMIL*, TRIA ANELIA

 Fakultas Farmasi Universitas Pancasila

 Jln. Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640.

 Diterima 20 Maret 2009,  Disetujui 24 Agustus 2009

Abstract: According to literatures, many plants of Papilionaceae family such as red bean (Phaseolus vulgaris L.), green bean (Phaseolus aureus Roxb.) and soybean (Glycine soja Sieb. & Zucc.) contain favonoid,  isofavonoid  and vitamin E. They all have antioxidant activity and are potential to reduce free radical. This research was aimed to identify secondary metabolites of crude powders and extracts of red bean, green bean and soybean. The bioactivity of each extracts was evaluated with Brine Shrimp Lethality Test (BSLT) method for preliminary assesment of toxicity. It was also evaluated for antioxidant activity using 1,1 difenil-2-pikrilhidrazil (DPPH) method. The results showed that red bean, green bean and soybean contain saponin, favonoid, steroid/triterpenoid, coumarin, and tannin. The result of BSLT showed that LC (Lethality Concentration 50) of red bean was 213.21 ppm; green bean 390.71 ppm;

and soybean 252.75 ppm. The result of DPPH tests showed that IC (Inhibition Concentration 50) of red bean was 47.54 ppm; green bean 95.08 ppm; and soybean 90.43 ppm.