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The needs of micro-scale power plant for small and middle residential or industry are beginning to increase, especially for continuous power generation in remote area, emergency standby and peak shaving. There are several types of micro-scale power plant commonly used, such as micro-steam turbine, micro-hydro turbine, micro-gas turbine (MGT), etc. Among those micro-scale power plant, MGT has the highest performance. Therefore, it is a promising technology to develop. In MGT, an automotive turbocharger could be used as the gas generator or and the turbine because it has a compact structure, light weight and commercially available.

In order to design a micro-gas turbine, it is necessary to find the optimum design of compressors such as the outlet compressor diameter, the combustion chamber and turbine. This paper describes the analysis of fluid flow in a micro-gas turbine that used an automotive turbocharger as the compressor and the turbine of MGT to generate electric power by using Liquefied Petroleum Gas (LPG). Three variations of compressor outlet diameter were developed and analyzed. The analysis shows that the compressor outlet diameter of 30 mm has a better velocity distribution, higher Mach number and higher turbulent intensity among other variants. The combustion chamber and the turbine also analyzed by using Computational Fluid Dynamics (CFD). The combustion chamber analysis indicates that the combustion of C3H8 and the air were mixed perfectly with maximum chamber temperature of 905.56 K. Meanwhile the turbine analysis indicates that the turbine rotated with a speed of 4087.96 m/s.

Interleukins and VEGF secretome of  human wharton’s Jelly mesenchymal stem cells-conditioned conditioned  (hwjmscs-CM) in different passages and CM) in different passages and CM) in different passages and CM) in different passages  and oxygen tensions

Wahyu Widowati1*, Halida Widyastuti2, Harry Murti2, Dian Ratih Laksmitawati3, Hanna Sari W Kusuma4, Rizal Rizal4, Ervi Afifah4, Sutiman B. Sumitro5, M. Aris Widodo6, Indra Bachtiar2
1Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia
2Stem Cell and Cancer Institute, Jl A Yani no 2 Pulo Mas, Jakarta 13210, Indonesia
3Faculty of Pharmacy, Pancasila University, Jagakarsa, Jakarta, Indonesia
4Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II no. 9, Bandung 40163, West Java, Indonesia
5Department of Biology, Faculty of Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
6Pharmacology Laboratory, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia
.
*Correspondence: wahyu_w60@yahoo.com Accepted: 20 Sep. 2017 Published online: 07 Nov. 2017

Abstract
Human mesenchymal stem cells (hMSCs) are powerful tools for tissue engineering in regenerative medicine. Human Wharton’s jelly mesenchymal stem cell-conditioned medium (hWJMSCs-CM) is medium harvested from hWJMSCs, which has been used in patients with myocardial infarction, heart failure and peripheral, vascular disease, containing various types of metabolites, proteins, mediator materials and it has properties anticancer, wound healing and anti-aging. In our previous research, hWJMSCs-CM cultured in normoxic and hypoxic condition exhibited anticancer activities toward cervical, ovarian, breast, liver, prostate and oral squamous cancer cells. The variety of the molecules in CM secretome is affected by types and numbers of cells, different condition, different passage, culture medium and condition. This research objective is to measure the growth factor and cytokines in hWJMSCs-CM cultured in normoxic and hypoxic condition (2.5%; 5% O2) from early passage (P4) and late passage (P8). The CM characteristic affected bioavailabilities and bioactivities. The hWJMSCs were isolated from umbilical cord of three women after full-term births. The secretome was measured including interleukin (IL)-1α, IL-6, IL-8 and vascular endothelial growth factor (VEGF) in both of normoxic and hypoxic oxygen tension, in early (P4) and late passage (P8). ). hWJMSCs secreted IL-1α in CM 2.75-4.85 pg/mL, IL-6 in CM 1,194.46-3,463.72 pg/mL, IL-8 in CM 2,049.63-6,322.30 pg/mL, VEGF in CM 24.96-51.86 pg/mL. These findings suggested that oxygen tension and passage condition affect cytokines and growth factor secretion, and revealed that hypoxic condition and late passage increase cytokines and growth factor level
Keywords: hWJMSCs, hypoxic, normoxic, cytokines, cconditioned medium, cancer

http://www.isisn.org/BR-14-2017/776-787-14(4)2017BR-1554.pdf

Conditioned medium from normoxia
(WJMSCs-norCM) and hypoxia-treated
WJMSCs (WJMSCs-hypoCM) in inhibiting
cancer cell proliferation
Wahyu Widowati a,*, Laura Wijaya b, Harry Murti b,
Halida Widyastuti b, Dwi Agustina b, Dian Ratih Laksmitawati c,
Nurul Fauziah d, Sutiman B. Sumitro e, M. Aris Widodo f,
Indra Bachtiar b,**
a Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java,
Indonesia
b Stem Cell and Cancer Institute, Jakarta, Indonesia
c Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
d Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
e Department of Biology, Faculty of Science, Brawijaya University, Malang, East Java, Indonesia
f Pharmacology Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia

Abstract Mesenchymal stem cells (MSCs) have unique properties, including high proliferation
rates, self-renewal, multilineage differentiation ability, wide multipotency, hypoimmunogenicity,
noninduction of teratomas, and anticancer properties. MSCs can be isolated from embryonic
and extraembryonic tissues as well as adult organs. Human Wharton’s jelly stem cellconditioned
medium possesses anticancer properties and inhibits the growth of solid tumors.
Lower oxygen concentration or hypoxic condition can increase the proliferation of MSCs, but
there are no differences in surface markers. We determined the osteocyte, chondrocyte,
and adipocyte differentiation of normoxic WJMSCs (nor-WJMSCs) and hypoxic 2.5%, hypoxic
5% (hypo-WJMSCs); from a different passage (P4 and P8), we determined the inhibitory
effect of WJMSCs-norCM and WJMSCs-hypoCM on the proliferation of human cancer cells
including cervical (HeLa), liver (HepG2), prostate (pc3), ovarian (skov3), and oral squamous
(hsc3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human

KEYWORDS
anticancer; conditioned medium; hypoxic; normoxic; Wharton’s jelly mesenchymal stemcells

http://dx.doi.org/10.1016/j.bgm.2014.08.008 

Phytochemical, Free Radical Scavenging and
Cytotoxic Assay of Cucumis Melo L. Extract and
β-Carotene
Wahyu Widowati
Maranatha Christian University, Faculty of Medicine, Bandung, Indonesia
Email: wahyu_w60@yahoo.com
Rachma Micho Widyanto, Dian Ratih Laksmitawati, Pande Putu Erawijantari, Laura Wijaya, and Ferry Sandra
Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia
Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
Stem Cell and Cancer Institute, Jakarta, Indonesia
Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
Email: {rahmamicho, dianratih.ffup, erawijantari, a.c.laura.w}@gmail.com, ferrysandra@yahoo.com

Abstract—Deaths from cancer worldwide are estimated to continue rising. Free radicals are toxic to cellular components. It is known that they cause DNA damage, contribute to DNA instability and mutation, thus favor carcinogenesis. This research was conducted to determine the activity of Cucumis melo extract and β-carotene in antioxidative and cytotoxic potencies. The research was done by examining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The cytotoxic potency was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
assay on HeLa, HepG2 and NIH3T3 cell lines. β-carotene exhibited more active DPPH free radical scavenging activity compared with C. melo extract. C. melo extract showed more active anti-cancer both in HeLa (IC50: 23.649 μg/mL) and HepG2 (IC50: 110.403 μg/mL) cancer cells. C. melo extract (IC50: 16,670.404 μg/mL) and β-carotene (IC50:50,645.994 μg/mL) had low cytotoxicity in NIH3T3 fibroblast. C. melo extract has lower antioxidant activity, but higher cytotoxic potency compared with β-carotene.
Index Terms—antioxidant, free radical, melon, cucumis melo, β-carotene, cancer, cytotoxic.

http://www.joaat.com/uploadfile/2015/0907/20150907105309257.pdf

Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear
Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed
Intracellular Reactive Oxygen Species Level of Peripheral Blood-derived
Endothelial Progenitor Cells
Wahyu Widowati1,*, Laura Wijaya2, Dian Ratih Laksmitawati3, Rahma Micho Widyanto4,
Pande Putu Erawijantari5, Nurul Fauziah5, Indra Bachtiar2, and Ferry Sandra6,7,*
1Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia
2Stem Cell and Cancer Institute, Jakarta 13210, Indonesia
3Faculty of Pharmacy, Pancasila University, Jakarta 12640, Indonesia
4Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia
5Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
6Faculty of Dentistry, Trisakti University, Jakarta 11440, Indonesia
7Prodia Clinical Laboratory, Jakarta 10430, Indonesia
Abstract − Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be
reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 μmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
Keywords − Tea flavonoids, Antioxidant, Endothelial progenitor cell, Differentiation, ROS, Apoptosis

http://dx.doi.org/10.20307/nps.2016.22.2.87 

https://www.researchgate.net/publication/305461566_Tea_Flavonoids_Induced_Differentiation_of_Peripheral_Blood-derived_Mononuclear_Cells_into_Peripheral_Blood-derived_Endothelial_Progenitor_Cells_and_Suppressed_Intracellular_Reactive_Oxygen_Species_Lev