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Publikasi Penelitian Dosen

VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR α-MANGOSTIN DALAM LARUTAN ORAL  EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) 
Abstrak

Ekstrak kulit buah manggis kaya akan antioksidan. α-Mangostin adalah komponen dengan efek antioksidan paling tinggi dalam kulit buah manggis. Larutan oral yang mengandung ekstrak kulit buah manggis perlu diuji penetapan kadar untuk pengujian mutunya. Penentuan kadar analit dalam sampel dengan matriks yang sangat kompleks dan jumlah analit sangat kecil, seperti α-mangostin dalam larutan oral, diperlukan metode yang selektif dan sensitif, yaitu kromatografi cair kinerja tinggi (KCKT). Pada penelitian ini dilakukan penetapan kadar α-mangostin dengan sistem KCKT fase balik dengan menggunakan fase diam oktadesilsilan (C18), fase gerak metanol-air (90:10), laju alir 1,0 mL/menit, dan detektor UV pada 316 nm. Waktu retensi α-mangostin adalah 9,622 menit. Puncak α-mangostin terpisah baik dengan resolusi sebesar 1,725. Linearitas metode berada pada rentang 1,67-5,01 bpj dengan koefisien korelasi 0,9986. Nilai simpangan baku relatif (SBR) sebesar 1,30%, perolehan kembali berada pada rentang 95,80-100,76% dengan nilai t hitungKata kunci: validasi metode, KCKT, α-mangostin, larutan oral, Garcinia mangostana L.

URL: 104-1606-1-PB_Alchemy.pdf

Penulis : ROS SUMARNY , Prof. Dr., MS, Apt [PDF File] didownload : 15 x

MEMPERKUAT LOYALITAS MELALUI KEPERCAYAAN KEPUASAN DAN KOMITMEN, STUDI PADA NASABAH PT. BANK CIMB NIAGA
Abstrak
Penulis : SRI WIDYASTUTI, DR, MM, MSI [PDF File] didownload : 5 x

Abstrak
Penulis : SRI WIDYASTUTI, DR, MM, MSI [PDF File] didownload : 8 x

ADSORPTION CAPACITY OF ULI BANANA (Musa x paradisiaca AAB) PEEL POWDER AS BIOSORBENT OF LEAD
Abstrak

Banana peel is one of biological material that has been used as biosorbent. Uli banana (Musa x paradisiaca AAB) peel is fairly abundant food waste. The aims of this study is to determine the adsorption capacity of uli bananas peel powder as biosorbent of lead solution. Uli banana peel was dried then grounded to powder, sieved until the particle size is less than 125 micron. The effect of pH and lead concentration was investigated. The optimum pH for removing the lead in aqueous solution is pH 4. The metal uptake capacity at pH 2, 4, 6, 8 are 0.5; 1.7; 1.5, 0.7 mg/g and at the lead concentration of 7.5, 10, 12.5, 15, 17.5, 20, 22.5 and 25 ppm are 1.1, 1.7, 1.7, 1.8, 1.9, 1.8, 2.0, 2.2 mg/g consecutively.

 

 

 

Keywords: biosorbent, banana peel, lead, uli banana

Penulis : LILIEK NURHIDAYATI , M.Si [PDF File] didownload : 6 x

Establishment Of Hyperuricemia Mouse Model With Oxonic Acid Potassium Salt And Essence Of Chicken.
Abstrak

Nowadays the exploration of anti-hyperuricemia herbs becomes more interesting, but making an animal model of hyperuricemia is still facing a problem due to a different uric acid metabolism between mice and human. The aim of this study was to investigate the effects of essence of chicken (EC) and urease inhibitor, oxonic acid potassium salt (PO) used singly or in combination on the concentration of serum uric acid in mice, to determine the optimal procedure, time of blood withdrawal and induction period and to evaluate the feasibility of this method to establish a mouse model of hyperuricemia. Determination of blood withdrawal time was as follows: DDY mice was given PO 250 mg/kgBW intra peritoneally and the blood was collected per hour to measure the plasma uric acid concentration. Determination of inductor combination and period of induction time were conducted by dividing mice into 6 groups: normal, PO 1 day, PO 3 days, PO and EC 1 day, PO and EC 3 days and EC 3 day and PO only on the third day. On the third day, the blood was collected and plasma uric acid was reacted with EHSPT (N-Ethyl-N-(2-hydroxy-3-Sulfopropil) m-Toluidine) and 4-AAP(4-Aminoantipyrine). The absorbance of color formation were measured by using spectrophotometer at 546 nm. The results showed that plasma uric acid was the highest at 2 hours after OA injection. The administration of both PO and EC could raise the uric acid compared with singly OA. Administration of EC and PO combination each day for 3 days’ consecutively could increase plasma uric acid in mice. In conclusion, the administration of chicken essence (EC) with oxonic acid potassium salt (PO) for 3 days significantly increased the plasma uric acid. Thus, this procedure could be used as a suitable method to establish a mouse model of hyperuricemia.

Keywords: hyperuricemia model, potassium oxonic acid, essence of chicken.

Penulis : LILIEK NURHIDAYATI , M.Si [PDF File] didownload : 3 x

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