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Publikasi Penelitian Dosen

Abstrak

Interleukins and VEGF secretome of  human wharton’s Jelly mesenchymal stem cells-conditioned conditioned  (hwjmscs-CM) in different passages and CM) in different passages and CM) in different passages and CM) in different passages  and oxygen tensions


Wahyu Widowati1*, Halida Widyastuti2, Harry Murti2, Dian Ratih Laksmitawati3, Hanna Sari W Kusuma4, Rizal Rizal4, Ervi Afifah4, Sutiman B. Sumitro5, M. Aris Widodo6, Indra Bachtiar2
1Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia
2Stem Cell and Cancer Institute, Jl A Yani no 2 Pulo Mas, Jakarta 13210, Indonesia
3Faculty of Pharmacy, Pancasila University, Jagakarsa, Jakarta, Indonesia
4Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II no. 9, Bandung 40163, West Java, Indonesia
5Department of Biology, Faculty of Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
6Pharmacology Laboratory, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia
.
*Correspondence: wahyu_w60@yahoo.com Accepted: 20 Sep. 2017 Published online: 07 Nov. 2017

Abstract
Human mesenchymal stem cells (hMSCs) are powerful tools for tissue engineering in regenerative medicine. Human Wharton’s jelly mesenchymal stem cell-conditioned medium (hWJMSCs-CM) is medium harvested from hWJMSCs, which has been used in patients with myocardial infarction, heart failure and peripheral, vascular disease, containing various types of metabolites, proteins, mediator materials and it has properties anticancer, wound healing and anti-aging. In our previous research, hWJMSCs-CM cultured in normoxic and hypoxic condition exhibited anticancer activities toward cervical, ovarian, breast, liver, prostate and oral squamous cancer cells. The variety of the molecules in CM secretome is affected by types and numbers of cells, different condition, different passage, culture medium and condition. This research objective is to measure the growth factor and cytokines in hWJMSCs-CM cultured in normoxic and hypoxic condition (2.5%; 5% O2) from early passage (P4) and late passage (P8). The CM characteristic affected bioavailabilities and bioactivities. The hWJMSCs were isolated from umbilical cord of three women after full-term births. The secretome was measured including interleukin (IL)-1α, IL-6, IL-8 and vascular endothelial growth factor (VEGF) in both of normoxic and hypoxic oxygen tension, in early (P4) and late passage (P8). ). hWJMSCs secreted IL-1α in CM 2.75-4.85 pg/mL, IL-6 in CM 1,194.46-3,463.72 pg/mL, IL-8 in CM 2,049.63-6,322.30 pg/mL, VEGF in CM 24.96-51.86 pg/mL. These findings suggested that oxygen tension and passage condition affect cytokines and growth factor secretion, and revealed that hypoxic condition and late passage increase cytokines and growth factor level
Keywords: hWJMSCs, hypoxic, normoxic, cytokines, cconditioned medium, cancer

http://www.isisn.org/BR-14-2017/776-787-14(4)2017BR-1554.pdf

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 0 x

Conditioned medium from normoxia (WJMSCs-norCM) and hypoxia-treated WJMSCs (WJMSCs-hypoCM) in inhibiting cancer cell proliferation
Abstrak

Conditioned medium from normoxia
(WJMSCs-norCM) and hypoxia-treated
WJMSCs (WJMSCs-hypoCM) in inhibiting
cancer cell proliferation
Wahyu Widowati a,*, Laura Wijaya b, Harry Murti b,
Halida Widyastuti b, Dwi Agustina b, Dian Ratih Laksmitawati c,
Nurul Fauziah d, Sutiman B. Sumitro e, M. Aris Widodo f,
Indra Bachtiar b,**
a Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java,
Indonesia
b Stem Cell and Cancer Institute, Jakarta, Indonesia
c Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
d Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
e Department of Biology, Faculty of Science, Brawijaya University, Malang, East Java, Indonesia
f Pharmacology Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia

 

Abstract Mesenchymal stem cells (MSCs) have unique properties, including high proliferation
rates, self-renewal, multilineage differentiation ability, wide multipotency, hypoimmunogenicity,
noninduction of teratomas, and anticancer properties. MSCs can be isolated from embryonic
and extraembryonic tissues as well as adult organs. Human Wharton’s jelly stem cellconditioned
medium possesses anticancer properties and inhibits the growth of solid tumors.
Lower oxygen concentration or hypoxic condition can increase the proliferation of MSCs, but
there are no differences in surface markers. We determined the osteocyte, chondrocyte,
and adipocyte differentiation of normoxic WJMSCs (nor-WJMSCs) and hypoxic 2.5%, hypoxic
5% (hypo-WJMSCs); from a different passage (P4 and P8), we determined the inhibitory
effect of WJMSCs-norCM and WJMSCs-hypoCM on the proliferation of human cancer cells
including cervical (HeLa), liver (HepG2), prostate (pc3), ovarian (skov3), and oral squamous
(hsc3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human

KEYWORDS
anticancer; conditioned medium; hypoxic; normoxic; Wharton’s jelly mesenchymal stemcells

http://dx.doi.org/10.1016/j.bgm.2014.08.008 

http://www.sciencedirect.com/science/article/pii/S2214024714000914

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 0 x

Phytochemical, Free Radical Scavenging and Cytotoxic Assay of Cucumis Melo L. Extract and β-Carotene
Abstrak

Phytochemical, Free Radical Scavenging and
Cytotoxic Assay of Cucumis Melo L. Extract and
β-Carotene
Wahyu Widowati
Maranatha Christian University, Faculty of Medicine, Bandung, Indonesia
Email: wahyu_w60@yahoo.com
Rachma Micho Widyanto, Dian Ratih Laksmitawati, Pande Putu Erawijantari, Laura Wijaya, and Ferry Sandra
Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia
Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
Stem Cell and Cancer Institute, Jakarta, Indonesia
Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
Email: {rahmamicho, dianratih.ffup, erawijantari, a.c.laura.w}@gmail.com, ferrysandra@yahoo.com

Abstract—Deaths from cancer worldwide are estimated to continue rising. Free radicals are toxic to cellular components. It is known that they cause DNA damage, contribute to DNA instability and mutation, thus favor carcinogenesis. This research was conducted to determine the activity of Cucumis melo extract and β-carotene in antioxidative and cytotoxic potencies. The research was done by examining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The cytotoxic potency was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
assay on HeLa, HepG2 and NIH3T3 cell lines. β-carotene exhibited more active DPPH free radical scavenging activity compared with C. melo extract. C. melo extract showed more active anti-cancer both in HeLa (IC50: 23.649 μg/mL) and HepG2 (IC50: 110.403 μg/mL) cancer cells. C. melo extract (IC50: 16,670.404 μg/mL) and β-carotene (IC50:50,645.994 μg/mL) had low cytotoxicity in NIH3T3 fibroblast. C. melo extract has lower antioxidant activity, but higher cytotoxic potency compared with β-carotene.
Index Terms—antioxidant, free radical, melon, cucumis melo, β-carotene, cancer, cytotoxic.

http://www.joaat.com/uploadfile/2015/0907/20150907105309257.pdf

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 0 x

Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed Intracellular Reactive Oxygen Species Lev
Abstrak

Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear
Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed
Intracellular Reactive Oxygen Species Level of Peripheral Blood-derived
Endothelial Progenitor Cells
Wahyu Widowati1,*, Laura Wijaya2, Dian Ratih Laksmitawati3, Rahma Micho Widyanto4,
Pande Putu Erawijantari5, Nurul Fauziah5, Indra Bachtiar2, and Ferry Sandra6,7,*
1Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia
2Stem Cell and Cancer Institute, Jakarta 13210, Indonesia
3Faculty of Pharmacy, Pancasila University, Jakarta 12640, Indonesia
4Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia
5Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
6Faculty of Dentistry, Trisakti University, Jakarta 11440, Indonesia
7Prodia Clinical Laboratory, Jakarta 10430, Indonesia
Abstract − Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be
reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 μmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
Keywords − Tea flavonoids, Antioxidant, Endothelial progenitor cell, Differentiation, ROS, Apoptosis

 

http://dx.doi.org/10.20307/nps.2016.22.2.87 

 

https://www.researchgate.net/publication/305461566_Tea_Flavonoids_Induced_Differentiation_of_Peripheral_Blood-derived_Mononuclear_Cells_into_Peripheral_Blood-derived_Endothelial_Progenitor_Cells_and_Suppressed_Intracellular_Reactive_Oxygen_Species_Lev

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 0 x

In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW264.7 murine macrophage cell line
Abstrak

In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW264.7 murine macrophage cell line
Ni Made Dwi Sandhiutami 1, Moordiani Moordiani 1, Dian Ratih Laksmitawati 1, Nurul Fauziah 2, Maesaroh Maesaroh 2, Wahyu Widowati 3*
1 Faculty of Pharmacy, University of Pancasila, Jl. Srenseng Sawah, Jagakarsa, Jakarta 12640, Indonesia
2 Bimolecular and Biomedical Research Center, Aretha Medika Utama,Jl. Babakan Jeruk 2 no 9, Bandung 40163, Indonesia
3 Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Suria Sumantri no 65 Bandung 40164, Indonesia

A B S T R A C T

Objective(s): Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer’s and various cancer. Indonesian cassia (Cinnamomum burmannil C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an anti-inflammatory. This study was conducted to determine the anti-inflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW264.7 cell line.
Materials and Methods: The cytotoxic assay of coumarin and ICE against RAW264.7 cells was conducted using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW 267.4 macrophages cells to measure inhibitory activity of compound and ISEon production of nitric oxide (NO), prostaglandin E2 (PGE2), and also cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α.
Results: Coumarin 10 μM and ICE 10 μg/ml were nontoxic to the RAW264.7 cells. Both of coumarin and ICE were capable to reduce the PGE2, TNF-α, NO, IL-6, and IL-β level in LPS-induced RAW264.7 cells. Coumarin had higher activity to decrease PGE2 and TNF-α, whilst ICE had higher activity to inhibit NO, IL-6, and IL-β levels.
Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE2 and NO along with pro-inflammatory cytokines TNF-α, IL-6, IL-1β production.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243982/pdf/IJBMS-20-99.pdf

Penulis : DIAN RATIH L , Dr., M.Biomed., [PDF File] didownload : 0 x

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