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Publikasi Penelitian Dosen

PHYTOCHEMICAL SCREENING AND ANTIMALARIAL ACTIVITY OF SOME PLANTS TRADITIONALLY USED IN INDONESIA
Abstrak

PHYTOCHEMICAL SCREENING AND ANTIMALARIAL ACTIVITY OF SOME PLANTS TRADITIONALLY USED IN INDONESIA

 

Syamsudin Abdillah, Risma Marisi Tambunan, Yunahara Farida, Ni Made Dwi Sandhiutami, Rita Marleta Dewi

 

Objectives : To evaluate ethanolic extracts of phytochemical screening, in vitro and in vivo antiplasmodial activities of 15 plants used as antimalarial in Sei Kepayang, North Sumatra.

Methods : Extraction was done through meceration with 70% ethanol and screened againts chemical content, in vitro test anti-plasmodium againts Plasmodium falciparm 3D7 strain and in vivo test in mice infected Plasmodium berghei

Result : The results showed that the plant extract contained a group of saponins, flavonoids,alkaloids,quinone, sterols, triterpene, tannins and cumarins. However, extract of Momordica charantia, Carica papaya, Garcinia atroviridas, Alstonia scholaris, Smallanthus sonchifolia and Cassia siamea had strong anti-plasmodium activity both in vitro and in vivo

Conclusions : In vitro and in vivo antiplasmodial activities of 15 plants are used as antimalarial in Sei Kepayang, North Sumatra. All the plants have in vitro and in vivo anti-plasmodium activity except Orthosiphon staminueus and Luffa cylindrica (ED50 > 1000 mg/kg body weight and IC50 > 100 µg/mL, respectively).

 

Keyword :

phytochemical screening, Plasmodium falciparm strain  3D7, Plasmodium berghei, ethanolic extracts

Penulis : NI MADE DWI SANDHIUTAMI, S.SI,M.KES.,APT [PDF File] didownload : 3 x

JERUK PURUT (Citrus hystrix D.C) JUICE REDUCED BLOOD GLUCOSE LEVELS AND HISTOPATHOLOGY OF INDUCED-ALLOXAN TETRAHYDRAT IN MICE
Abstrak

ABSTRACT

 

 

JERUK PURUT (Citrus hystrix D.C) JUICE REDUCED BLOOD GLUCOSE LEVELS AND HISTOPATHOLOGY OF INDUCED-ALLOXAN TETRAHYDRAT IN MICE

 

 

Ni Made Dwi Sandhiutami,S.Si.,M.Kes.,Apt.; Dra. Lestari Rahayu,MS.,Apt.;

 Prapanca Fitria Sutomo, S.Farm.,Apt

 

       Faculty of Pharmacy, Pancasila University

 

Jeruk purut (Citrus hystrix D.C) has shown potential as antidiabetic through the inhibition of of enzyme α-glucosidase in vitro. This study aims to determine the effect of jeruk purut juice for the reduction of blood glucose levels in vivo as well as the changes in pancreatic β cells in mice. In the test, 24 mice were divided into 4 groups of 6 mice each: a normal group (I), a negative control group (II), a positive control group treated with acarbose (III) and a final group had been treated with dose 12,5 g/kg of body weight administered orally (IV). Mice glucose blood levels were observed during regular conditions and after an intraperitoneal alloxan induction of 250 mg/kg of body weight which made the mice hyperglicemic. Mice were in hyperglicemic conditions on the 3rd day. The preparation given every day and blood glucose levels were observed on the 3rd, 6th, 9th, and 12nd day. Data was analyzed with one-way ANOVA and continued by LSD test. The result showed that a jeruk purut juice dose 12,5 g/kg of body weight reduce the blood glucose levels in hyperglycemic mice and increased the pancreatic β cell and its effectiveness is equal to acarbose.

 

Keywords: Diabetes, Citrus hystrix D.C, blood glucose level, alloxan tetrahydrate, acarbose, pancreatic β cells

Penulis : NI MADE DWI SANDHIUTAMI, S.SI,M.KES.,APT [PDF File] didownload : 3 x

ANTIOXIDANT EFFECT OF ETHANOL EXTRACT FROM RAMBUTAN PEEL (Nephelium lappaceum L.) ON MALONDIALDEHID CONTENT AND SUPEROXIDE DISMUTASE ACTIVITY IN MICE
Abstrak

ANTIOXIDANT EFFECT OF  ETHANOL EXTRACT FROM RAMBUTAN PEEL (Nephelium lappaceum L.) ON MALONDIALDEHID CONTENT AND SUPEROXIDE DISMUTASE ACTIVITY IN MICE

 

Ni Made Dwi Sandhiutami, S.Si, M.Kes., Apt, Dra. Lestari Rahayu, M.S.,Apt.,                       Nisa Yaumil Nur Azilia

 

Faculty of Pharmacy, Pancasila University

Email : dwi_sandhiutami@yahoo.com

 

Rambutan peel from Sapindaceae family is known for its antioxidant activity. Rambutan peel contains group of fenolic compounds such as flavonoid reported to have an important role against antioxidant activity. The purpose of this research is to investigate antioxidant effect of ethanol extract from rambutan peel in mice with measurement of plasma MDA using TBA test method, and measurement of red blood cells SOD activity using Adenochrom Assay method. Mice were given rambutan peel etanol extract for 7 days, and swim exercise for 65 minutes on day 7. Total 36 mice was divided into 6 groups: normal group, negative control group, positive control group of vitamin C, dose treatment group 21 mg/kgBB, a dose of 42 mg/BBkg, and a dose of 84 mg/kgBB, for 7 days . The results of antioxidant activity measurement in vivo, plasma MDA levels and blood cell SOD activity obtained for the normal group is 1.6524 ± 0.0793nmol/mL and 88.89 ± 6.54 U/mL, negative control group is 0.1196 ± 6.3399 nmol/mL and 45.03 ± 6.04 U/mL, the positive control group of vitamin C is 1.3024 ± 0.1321 nmol/mL and 85,18±9,29 U/mL, 21mg/kgBB dose group is 3.7833 ± 0.2985 nmol/mLand 63.49 ± 7.24 U/mL, 42 mg/kgBB dose group is 2.2628 ± 0.3801nmol/mL and 82.01 ± 3.71 U/mL and 84 mg/kgBB dose group is 4.6794 ± 0.3501 nmol/mL and 52.91 ± 6.86 U/mL. In conclusion, all doses can decrease the plasma MDA levels and increase the SOD activity of red blood cell. SOD activity of dose treatment group 42 mg/kgBB is not significantly different with the positive control group of vitamin C.

 

Keywords : Rambutan’s peel, antioxidant, MDA, SOD.

Penulis : NI MADE DWI SANDHIUTAMI, S.SI,M.KES.,APT [PDF File] didownload : 2 x

UJI TOKSISITAS AKUT, AKTIVITAS ANTIOKSIDAN IN VITRO DAN EFEK REBUSAN BUNGA KEMBOJA MERAH (Plumeria rubra L.) TERHADAP KADAR MALONDIALDEHID
Abstrak

UJI TOKSISITAS AKUT, AKTIVITAS ANTIOKSIDAN IN VITRO DAN EFEK REBUSAN BUNGA KEMBOJA MERAH  (Plumeria rubra L.) TERHADAP KADAR MALONDIALDEHID

 

NI MADE DWI SANDHIUTAMI*, LESTARI RAHAYU

Fakultas Farmasi Universitas Pancasila

 

Abstrak : Antioksidan merupakan senyawa yang mampu menghambat reaksi radikal bebas dalam tubuh. Penelitian ini bertujuan untuk mendapatkan nilai LD50, mengetahui aktivitas antioksidan secara in vitro dan efek rebusan bunga kemboja merah (Plumeria rubra L.) terhadap kadar malondialdehid. Nilai LD50 didapatkan dengan menggunakan metode Weil. Uji aktivitas antioksidan secara in vitro dilakukan dengan metode DPPH yang berdasarkan reaksi penangkapan hidrogen dari antioksidan dan secara in vivo dengan melihat efek rebusan bunga kemboja merah dilakukan dengan mengukur kadar MDA plasma. Pemeriksaan kadar MDA dilakukan dengan metode Tiobarbituric acid reactivity test. Dalam pengujian in vivo, 30 ekor mencit dibagi menjadi 6 kelompok yaitu  kelompok normal, kelompok kontrol negatif, kelompok kontrol positif, dan 3 kelompok perlakuan dengan variasi dosis rebusan bunga kemboja merah. Hasil pengujian diperoleh nilai LD50 rebusan bunga kemboja adalah > 15 g/kgBB. Aktivitas antioksidan secara in vitro menunjukkan bahwa nilai IC50 rebusan bunga kemboja merah sebesar 36,96 µg/mL. Aktivitas antioksidan rebusan bunga kemboja merah lebih kecil daripada vitamin C (IC50 5,89 µg/mL). Efek rebusan bunga kemboja merah terhadap kadar MDA  menunjukkan rata-rata kadar MDA kelompok normal sebesar 1,66 nmol/mL, kelompok kontrol negatif  sebesar 5,67 nmol/mL, kelompok kontrol positif sebesar 1,45 nmol/mL, kelompok perlakuan yang diberikan rebusan bunga kemboja merah dengan dosis 0,39 g/kg BB sebesar 4,32 nmol/mL, dosis 0,78 g/kg BB sebesar 3,68 nmol/mL, dan dosis 1,56 g/kg BB sebesar 1,13 nmol/mL. Kelompok perlakuan yang diberikan rebusan bunga kemboja merah dosis 1,56 g/kg BB tidak berbeda bermakna dengan kelompok kontrol positif.

 

Kata kunci : antioksidan, rebusan bunga kemboja merah, DPPH, IC50, MDA 

 

Penulis : NI MADE DWI SANDHIUTAMI, S.SI,M.KES.,APT [PDF File] didownload : 3 x

UJI AKTIVITAS ANTIOKSIDAN REBUSAN DAUN SAMBANG GETIH (Hemigraphis bicolor Boerl.) SECARA IN VIV0
Abstrak

UJI AKTIVITAS ANTIOKSIDAN

REBUSAN DAUN SAMBANG GETIH

(Hemigraphis bicolor Boerl.) SECARA  IN VIV0

 

Dra. Lestari Rahayu, M.S., Apt., Ni Made Dwi S, S.Si, M.Kes., Apt.,

Lili Yusnita Sari

Fakultas Farmasi, Universitas Pancasila, Jakarta.

ABSTRAK

Latar Belakang : Aktivitas fisik yang berlebih dapat menyebabkan terjadinya stres oksidatif pada manusia dan pada mencit. Stres oksidatif adalah suatu keadaan dimana produksi radikal bebas melebihi produksi antioksidan. Antioksidan merupakan seperangkat sistem pertahanan yang dapat melindungi tubuh dari kerusakan oleh radikal bebas.

Tujuan : Untuk mengetahui aktivitas antioksidan rebusan daun sambang getih secara in vivo dengan mengukur kadar MDA plasma mencit yang diberi perlakuan dengan cara pemberian rebusan daun sambang getih selama 7 hari dan perenangan selama 55 menit pada hari ke-7.

Metode : Pengujian aktivitas antioksidan secara in vivo dengan mengukur kadar MDA plasma dilakukan dengan cara pembagian 30 ekor mencit menjadi 6 kelompok yaitu kelompok normal, kelompok kontrol negatif, kelompok kontrol positif vitamin C dosis 6,5 mg/kgBB, kelompok perlakuan dosis 1,95 g/kgBB, kelompok perlakuan dosis  3,9 g/kgBB, kelompok perlakuan dosis 7,8 g/kgBB, selama 7 hari dan dan perenangan selama 55 menit pada hari ke-7.

Hasil :  Pengukuran aktivitas antioksidan secara in vivo didapat kadar MDA plasma kelompok normal sebesar 1,66±0,50 nmol/mL, kelompok kontrol negatif sebesar 5,67±0,30 nmol/mL, kelompok kontrol positif vitamin C dosis 6,5 mg/kgBB sebesar 1,45±0,58 nmol/mL, kelompok perlakuan dosis 1,95 g/kgBB sebesar 1,99±0,21 nmol/mL, kelompok perlakuan dosis 3,9 g/kgBB sebesar 1,46±0,18 nmol/mL dan kelompok perlakuan dosis 7,8 g/kgBB sebesar  0,85±0,19 nmol/mL. Kadar MDA plasma kelompok perlakuan dosis 3,9 g/kgBB tidak berbeda bermakna dengan kelompok kontrol positif vitamin C dosis 6,5 mg/kgBB.

Kesimpulan : Uji aktivitas antioksidan rebusan daun sambang getih menunjukkan bahwa kadar MDA plasma pada ketiga kelompok perlakuan dosis tidak berbeda bermakna dengan kelompok kontrol positif vitamin C dosis 6,5 mg/kgBB.

 

Kata kunci : Antioksidan, sambang getih, MDA

Penulis : NI MADE DWI SANDHIUTAMI, S.SI,M.KES.,APT [PDF File] didownload : 3 x

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